FungiQuant: A broad-coverage fungal quantitative real-time PCR assay
نویسندگان
چکیده
منابع مشابه
Real-time PCR assay for quantitative mismatch detection.
We describe here a quantitative real-time PCR assay for the detection of single-base-pair differences that does not require fluorescently labeled gene-specific probes or complicated primer combinations. Following PCR or RT-PCR of a gene segment that may contain allele-specific differences, 100 pg amplified product are used for a real-time PCR with allele-specific primers and SYBR Green. The use...
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ژورنال
عنوان ژورنال: BMC Microbiology
سال: 2012
ISSN: 1471-2180
DOI: 10.1186/1471-2180-12-255